VanSaun2, Lynn M. Matrisian2, D. Lee Gorden2 1 Department of Surgery, St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea Republic, 2 Department of Cancer Biology, Vanderbilt University, Nashville, Tennessee, USA Purpose: Pro-inflammatory processes of the early postoperative states may induce peritoneal metastases in patients with advanced diseases. To identify that wound selleck kinase inhibitor healing response

after an abdominal incision leads to increased MMP-9 activity locally, therefore providing a favorable environment for peritoneal metastasis. Increased MMP9 in a post-operative injury setting increases the number and severity of peritoneal metastasis when compared to mice without wounds. Methods: Eighteen C57bl/6 J male

mice were obtained at 8 weeks of age. Metastatic tumors were initiated using a peritoneal injection model with syngeneic MC38 murine colon cancer cells. Peritoneal ACP-196 supplier injections were performed into the intraperitoneum at right lower quadrant area via 25G syringe. A 1.5 cm upper midline incision was made in the abdominal wall to recapitulate the postoperative wound model. The abdominal wall was closed by a continuous 4-0 prolene suture with 5 stitches. Mice were sacrificed at various time points. And we observed the rate of the peritoneal metastasis from each group. Results: By making incision into the abdominal wall, we induced inflammation of the mouse and observed the incidence of the peritoneal metastasis was increased(Fig.1). Early stage of wound healing process

increases pro-inflammatory cytokines and number of inflammatory cells in the peritoneum, and this leads to increase pro-MMP9 proteins. And the inflammatory process which initiated by the wound, in turn, increased the proliferation of the mesothelial cells and provoked expression of the inflammatory cells and increased parietal peritoneal metastasis. Conclusion: stage of wound healing process increases pro-inflammatory cytokines and number of inflammatory cells in the peritoneum, and this leads to increase pro-MMP9 proteins. So the increased pro-MMP9 proteins play a key role on the growth and progressions of cancer cells in peritoneal not metastasis. Figure 1. Poster No. 87 Cytokine-Mediated Activation of Gr-1 + Inflammatory Cells and Macrophages in Squamous Cell Carcinoma towards a Tumor-Supporting Pro-Invasive and Pro-Angiogenic Phenotype Nina Linde 1 , Dennis Dauscher1, Margareta M. Mueller1 1 Group Tumor and Microenvironment, German Cancer Research Center, Heidelberg, Germany Inflammatory cells have been widely accepted to contribute to tumor formation and progression. In a HaCaT model for human squamous cell carcinoma (SCC) of the skin, we have observed that infiltration of inflammatory cells does not only promote tumorigenesis but is indispensable for persisten angiogenesis and the development of malignant tumors.