SSD received fellowship from Department of Biotechnology (DBT), Government of India. This experimental work in S. album in the author’s laboratory was supported under the project – Prospecting of novel genes and molecules of S. album L. (NGM), sponsored

by DBT, Government of India. “
“Cefpodoxime proxetil (CP) is an orally absorbed, broad spectrum, third generation cephalosporin ester. This prodrug ester is hydrolyzed in vivo into its active metabolite, cefpodoxime. In human, the absolute bioavailability of CP administered as a 130 mg tablet (equivalent 100 mg of cefpodoxime) is about 50%. 1 However, the high solubility, chemical and enzymatic stability, and absorption profile of CP in acidic pH values of stomach, points to the potential of a gastroretentive (GR) dosage form

in altering the absorption profile of CP. 2 Mucoadhesive drug delivery systems for its potential http://www.selleckchem.com/products/ABT-737.html CX-5461 as optimize localized drug delivery, by retaining a dosage form at the site of action or systemic delivery, by retaining a formulation in intimate contact with the absorption site. 3 and 4 Despite the mucoadhesion, the advantage of using microspheres as oral mucoadhesive drug delivery system is that the small size microspheres can be trapped in the reductus of the stomach and stay there longer. Besides, when poorly soluble drugs were loaded in the mucoadhesive microspheres, there were either adsorbed at the surface of the microspheres or highly dispersed in the inner part of the microspheres which may help enhance the solubility of the drugs, results in improved bioavailability. 5 Chitosan (CS), a cationic polymer and an interesting material for microparticulate systems because

of its good mucoadhesive and biodegradable properties. 6 It is well established that traditional experimentation involves a good deal of efforts and time especially when complex formulations secondly are to be developed. In addition to the art of formulation, the technique of factorial design is an efficient method of indicating the relative significance of a number of variables and their interactions. 7 The objective of the present work is to improve the oral bioavailability of CP by formulating gastroretentive mucoadhesive microspheres which will provide protection from intestinal milieu using CS and to characterize for in vitro and in vivo parameters. A 32 full factorial design (two variables in three levels) was employed to evaluate the combined effect of the selected independent variables: CP to CS ratio (A) and amount of glutaraldehyde (GA) (B) on dependent variables such as drug entrapment efficiency, swelling index, percentage mucoadhesion and time for 50% drug dissolution (t50). Cefpodoxime proxetil was received as a gift sample from Orchid Chemicals and Pharmaceuticals Ltd, Chennai. Chitosan (≥75% deacetylated) obtained from Sigma Aldrich (Mumbai, India). Dioctyl sodium sulfo succinate (DOSS), petroleum ether (S.