Moreover, HABP 30987 showed larger inhibitory effect at the smaller concentration tested in this assay. HABP 30979 inhibited invasion of both cell types by a larger or even higher percentage than the ones shown by the colchicine and Cytochalasin controls. This HABP showed a dose-dependent inhibitory effect on both cells, achieving the highest inhibitory percentage

at 200 μM. The ability of Rv0679c peptides to inhibit M. tuberculosis invasion of target cells suggests that active and specific 17DMAG mw binding to cell surface receptors prevents entry of M. tuberculosis through this invasion pathway. Such notion is further supported by the results of internalization assays carried out with peptide-coated latex beads selleck chemicals llc and epithelial cells, where peptide-coated beads were more actively internalized than uncoated beads. Particularly HABP 30979, which was the strongest invasion

inhibitor, displayed the highest internalization percentages. On the other hand, the large inhibition percentages obtained with phagocytic cells in comparison to the ones obtained with epithelial cells might be explained by the cooperativity phenomenon observed in saturation assays with CB-5083 solubility dmso the phagocytic cell line, since the amount of peptide that binds to surface receptors is proportional to the probability of forming more stable ligand-receptor complexes and thereby of restricting mycobacterial entrance. Furthermore, since some HABPs showed high binding activity to one cell type but low binding activity to the other one, it could be suggested that peptide binding activity depends on specific receptor molecules expressed on each cell type. Consequently, binding of Rv0679c HABPs with high activity to both cell lines could be due to the presence of the same receptor on both cell types or to different receptors Farnesyltransferase with similar characteristics. To date, no structural model has been reported for this protein. Therefore, CD assays were

conducted in order to determine whether there was a relationship between the secondary structure of Rv0679c peptides and their binding ability or in their ability to inhibit mycobacterial invasion. CD spectrum data suggested that the secondary structure of HABP 30979 and 30985 was formed by α-helix and random coil elements, while peptides 30982 to 30984 and HABPs 30986 and 30987 showed undefined structural features. The results indicate that there is not a direct relationship between the structure of HABPs and their ability to binding to target cells. Interestingly, the results obtained in this study showed that the HABPs that inhibited mycobacterial invasion to target cells more efficiently were also the ones that showed the larger internalization percentages, therefore suggesting that Rv0679c HABPs promote entry of pathogenic M. tuberculosis into host cells.