Lane 1: negative control (ddH2O); lane 2: negative control (empty, self-ligated vector); lane 3: positive control (GAPDH); lane 4: marker; lanes 5–12: 1-8# transformation. (TIFF 147 KB) Additional file 2: Sequence analysis. (PDF 500 KB) References 1. Forner A, Llovet JM, Bruix J: Hepatocellular carcinoma. Lancet Angiogenesis inhibitor 2012, 379:1245–1255.PubMedCrossRef 2. Pang RW, Joh JW, Johnson PJ, Monden M, Pawlik TM, Poon RT: Biology of hepatocellular carcinoma. Ann Surg Oncol 2008, 15:962–971.PubMedCrossRef 3. Wu XZ, Xie GR, Chen D: Hypoxia and hepatocellular carcinoma: the therapeutic target for hepatocellular carcinoma. J Gastroenterol

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