Depending on the environment, the metal release from stainless steel is also influenced by other processes including active corrosion and protonation [4], [11] and [14]. The dynamic exchange of proteins between the surface and the solution is important for the metal release process, and depends on various factors including protein concentration and agitation [16]. Surface adsorption

is influenced by the surface charge of stainless steel and of adsorbed species [17], which results in electrostatic (EL) forces (repelling or attractive) [18]. Non-polar (electrodynamic, or Lifshitz-van der Waals – LW), and polar (electron–donor, electron–acceptor, or Lewis acid–base – AB) interactions are both important for any surface adsorption [17], [18] and [19]. These properties can be determined via contact angle measurements using liquids of different and known

GSK 3 inhibitor surface energy components [18]. Dehydration of water at the surface and changes in protein conformation where the driving force is a net gain in entropy, are important in the case of BSA adsorption. This process takes place even if the polarity is the same as the stainless steel surface [20] and [21]. The surface charge is important for the adsorption of proteins (especially for small, hard proteins), since one of the driving forces for protein adsorption on stainless steel is electrostatic [17], [20] and [21]. The zeta potential of massive stainless steel is commonly reported as negative at neutral pH and

the isoelectric point (IEP) was identified between pH 3 and 5 [4], [21], [22], [23] and [24]. Even significantly higher IEPs, between 6 and 8.5, have MK-2206 cost see more been reported for stainless steel particles [25] and [26] and massive sheet of stainless steel (predicted data) [27] and [28]. More positive IEPs of nano- and micron-sized stainless steel particles compared with massive sheet may be explained by differences in surface oxide speciation (such as composition, thickness, crystallinity, phase distribution, and catalytic properties) [29], [30] and [31]. No significant differences in IEP have been reported in the literature [32] for different pure metal particles and their corresponding bulk oxides. However, since all of these particles were treated in NaOH and HNO3 prior to the measurements, this might have influenced the results. Reported IEPs of bulk oxides and hydroxides of iron and chromium vary between 4.5 and 8.5 [33] and [34]. This is higher compared with measurements for massive stainless steel surface oxides made of similar constituents. Somewhat lower IEPs have been reported for several metals and alloys (e.g. stainless steel) [23] and [35] with thin surface oxides (as compared with bulk oxides). The lower IEPs of metals could possibly be explained by a mirror effect of electrons at the metallic interface adjacent to the thin surface oxide [36] and [37].