As mentioned in the previous section, tumor-derived oxysterols inhibit the expression of the chemokine receptor CCR7 on DCs undergoing maturation through the engagement of LXRα, as demonstrated by the acquired resistance to CCR7 inhibition in LXRα-silenced DCs exposed to synthetic and tumor-derived oxysterols in vitro, thereby dampening DC migration Linsitinib to draining LNs and the induction

of effective antitumor immune responses (Fig. 1B) [10]. As CCR7 drives DCs to secondary lymphoid organs [38], where they activate naïve T cells and B cells [39], CCR7 inhibition by oxysterols might represent one of the many immune escape mechanisms responsible for tumor growth [35]. This mechanism uniquely alters mature DC migration to secondary lymphoid organs in tumor-bearing IWR-1 chemical structure mice, as demonstrated by FITC skin-painting experiments in Lxrα−/− BM chimera mice, in which tumor-derived

oxysterols failed to inhibit FITC+ DC migration to draining LNs [10]. Consistent with this observation, tumor growth was found to be delayed in Lxrα−/− BM chimera mice as compared with WT BM chimera mice [10]. Noteworthy, tumors grew in Lxrβ−/− BM and WT BM chimeras (Russo et al. unpublished observations), suggesting that the overall function of LXRα and LXRβ isoforms in immune cells might be context-dependent. Immature DCs are involved in peripheral T-cell tolerance induction, as they express Sclareol low levels of

co-stimulatory molecules, and release anti-inflammatory cytokines such as IL-10 instead of IL-12 [20, 21]. Since it has been reported that LXR ligands induce CCR7 expression in immature DCs [27], it is possible to hypothesize that the presence of oxysterols within the tumor microenvironment could promote the migration of immature Ag-loaded DCs to secondary lymphoid organs, where they are likely to induce Ag-specific T-cell tolerance/anergy (Fig. 1C) [40]. This pathway could be further reinforced by the previously described LXRα- and LXRβ-dependent phagocytosis of apoptotic cells/bodies by immature DCs [19] (Fig. 1A). Since macrophages also phagocytose apoptotic cells/bodies in an LXRα- and LXRβ-dependent manner, we cannot rule out the possibility that macrophages participate in the tolerogenic presentation of tumor Ags to T cells. The above-described mechanisms could operate in a concerted action with the LXRα-induced CCR7 inhibition identified by our group (Fig. 1B) [10] to dampen the antitumor immune responses. Whether both mechanisms operate simultaneously within the tumor microenvironment deserves further investigation in appropriate models. The role of LXRβ activation in tumor-infiltrating Ag-specific T cells remains to be investigated. Tumor-derived oxysterols might be able to inhibit tumor-specific T cells [28] (Fig.