76% >0.99 Saccharomycotina Pichia ohmeri 102.31% >0.99 Saccharomycotina Saccharomycopsis crataegensis 80.98% >0.99 Saccharomycotina Stephanoascus ciferrii 85.84% >0.99 Mucoromycotina Absidia corymbifera 92.33% >0.99 Mucoromycotina Cunninghamella

bertholletiae 80.03% >0.99 Mucoromycotina Rhizopus microsporus 89.16% >0.99 Mucoromycotina Rhizopus oryzae 87.96% >0.99 Pezizomycotina Alternaria sp. 103.70% >0.99 Pezizomycotina Cladosporium cladosporioides 92.87% >0.99 Pezizomycotina Cytospora chrysosperma 100.50% >0.99 Pezizomycotina Endoconidioma sp. 89.93% >0.99 Pezizomycotina Geopora sp. 114.45% >0.99 Pezizomycotina Phoma herbarum 91.94% >0.99 Pezizomycotina Xanthomendoza galericulata 94.27% >0.99 Agaricomycotina Agaricus sp. 95.31% >0.99 Agaricomycotina Clavulina coralloides 99.59% >0.99 Selleckchem SB203580 Agaricomycotina Coprinus sp. 99.70% >0.99 Agaricomycotina Cortinarius sp. 102.68% >0.99 Agaricomycotina Hebeloma crustuliniforme group 91.06% >0.99 Agaricomycotina Melanogaster sp. 102.27% >0.99 Agaricomycotina Pleurotus ostreatus 102.71% >0.99 Agaricomycotina Rhizopogon sp. 107.04% >0.99 Agaricomycotina Sclerogaster xerophilus 92.17% >0.99

Agaricomycotina Sedecula pulvinata 92.26% >0.99 Agaricomycotina Tricholoma populinum 89.53% >0.99 Agaricomycotina Trichosporon asahii 78.03% >0.99 Agaricomycotina Trichosporon asteroides 82.66% >0.99 Agaricomycotina Trichosporon cutaneum 86.66% >0.99 Agaricomycotina Trichosporon LDE225 nmr dermatis 80.27% >0.99 Agaricomycotina Trichosporon faecale 84.05% >0.99 Agaricomycotina Trichosporon montevideense 77.43% >0.99 Agaricomycotina Trichosporon mucoides 82.87% >0.99 Agaricomycotina Trichosporon ovoides 105.59% >0.99 Pucciniomycotina Rhodotorula mucilaginosa 96.29% >0.99 Pucciniomycotina Rhodotorula slooffiae 99.94% >0.99 Agaricomycotina Lactarius sp. 86.76-89.03% >0.99 Bay 11-7085 Table 4 FungiQuant quantitative validation

results, obtained using pure plasmid standards and different mixed templates Templates tested Assay quantitative dynamic range Average reaction efficiency (SD) r 2 -value 10 μl Reaction       Plasmid standards-only 25 – 107 copies 91.80% (1.91%) >0.99 Plasmid standards plus 0.5 ng human DNA 25 – 107 copies 93.20% (0.70%) >0.99 Plasmid standards plus 1 ng human DNA 25 – 107 copies 97.02% (4.97%) >0.99 Plasmid standards plus 5 ng human DNA 25 – 107 copies 92.85% (1.33%) >0.99 Plasmid standards plus 10 ng human DNA 25 – 107 copies 91.21% (1.79%) >0.99 C. albicans DNA-only 10 fg – 10 ng 94.75% (2.33%) >0.98 C. albicans DNA plus 1 ng human DNA 10 fg – 10 ng 96.84% (1.93%) >0.99 5 μl Reaction       Plasmid standards-only 25 – 107 copies 92.17% (5.64%) >0.98 Plasmid standards plus 1 ng human DNA 25 – 107 copies 94.21% (2.92%) >0.99 Plasmid standards plus 10 ng human DNA 50 – 108 copies 92.64% (2.39%) >0.99 Table 5 Interpretation of FungiQuant results for detecting fungal DNA (i.e.