PCR products of sequentially related bacteriocins (colicins E2-9, selleck chemicals Ia-Ib, U-Y, 5–10) were verified using dideoxy terminator sequencing and amplification primers. Sequence analysis was carried out using Lasergene software (DNASTAR,

Inc., Madison, WI, USA). Screening for genes encoding virulence factors All 1181 E. coli strains were screened for the presence of genes for 17 different virulence factors (α-hly, afaI, aer, cnf1, sfa, pap, pCVD432, ial, lt, st, bfpA, eaeA, ipaH, iucC, fimA, stx1, stx2 and ehly). The primer pair sequences, PCR product lengths and PCR protocols used, were previously described [48–55]. Statistical analyses For statistical analysis of the incidence of bacteriocins and virulence factors, standard methods derived from the binomial distribution, including the two-tailed Fisher’s exact test corrected using the Bonferroni correction, were used. STATISTICA software, version 8.0 (StatSoft, Tulsa, OK, USA), was used for calculations. Distribution of virulence

factors and Thiazovivin nmr bacteriocin genes were determined using Correspondence Analysis (CA) and STATISTICA version 8.0. Availability https://www.selleckchem.com/products/rg-7112.html of supporting data The data set of 294 colicin gene sequences supporting the results of the article has been deposited in the GenBank/EMBL/DDBJ under accession numbers AB923519 – AB923812. Acknowledgments This work was supported by a grant from the Ministry of Health of the Czech Republic (NT13413-4/2012) to D.S. Electronic supplementary material Additional file 1: Table S1: Distribution of virulence determinants and bacteriocin genes among 1181 E. coli strains isolated from human fecal microflora. (DOCX 17 KB) Additional file 2: Table S2: DNA Primers used for PCR detection of colicin and microcin

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