Sexual morph link not equivicoally proven. Conidiomata dark brown to black, uniloculate, immersed in the host OICR-9429 in vitro becoming erumpent when mature. Paraphyses hyaline, septate, cylindrical, ends rounded. Conidiogenous cells hyaline, cylindrical, holoblastic. Conidia initially hyaline and aseptate, subovoid to ellipsoid-ovoid, with granular content, apex broadly rounded, remaining hyaline for a long time, becoming dark brown, 1–septate, thick-walled, base truncate or round, with longitudinal striations from apex to base. Notes: Lasiodiplodia was introduced by Clendenin (1896) with L. tubericola Ellis & Everh. as the type species; the current name is L. theobromae. Alves et al. (2008) provide a recent
description of the species which is widespread on many hosts. With the large number of hosts and its known morphological variability (Punithalingam 1980), it is possible that L. theobromae comprises a number of cryptic species (Alves et al. 2008). The type strain has questionably been linked to Botryodiplodia rhodina (Cooke) Arx, but this link has not been equivicoally proven (Alves et al. 2008; Phillips
et al. 2008). Further work selleck compound is required to establish the this website characters of the sexual morphs of Lasiodiplodia sensu stricto species, especially with the large number of potential cryptic species. Therefore no characters are given in the generic description; however, these are likely to be “Botryosphaeria”-like. The asexual morph can be distinguished by its distinctive striate brown conidia and numerous long paraphyses (Alves et al. 2008). It is hard to distinguish between species in this genus solely by morphology and analysis of EF1-α, β-tubulin and RPB2 gene sequences (Alves et al. 2008; Abdollahzadeh et al. 2010) or Genealogical Sorting Index (GSI), which has been used to resolve the asexual morph of Neofusicoccum
Dimethyl sulfoxide (Sakalidis et al. 2011), is needed to resolve species. Generic type: Lasiodiplodia theobromae (Pat.) Griffon & Maubl. Lasiodiplodia theobromae (Pat.) Griffon & Maubl., Bull. Soc. Mycol. Fr. 25: 57 (1909) MycoBank: MB188476 (Fig. 17) Fig. 17 Lasiodiplodia theobromae (MFLU 12–0760) a-b. Conidiomata on dead twigs. c-d. Section through conidioma. e Paraphyses. f Conidiogenous cells and young conidia. g-j. Mature conidia in two different focal planes to show the longitudinal striations. k. Spore germinated. l-m. Culture on PDA. Scale bars: c-d = 100 μm, e-j = 10 μm, k = 30 μm ≡ Botryodiplodia theobromae Pat., Bull. Soc.Mycol. Fr. 8: 136 (1892) = Diplodia gossypina Cooke, Grevillea 7: 95(1879) For other possible synonyms see Index Fungorum Saprobic on recently dead wood, often attached to tree, and endophytic. Sexual state not established. Conidiomata dark brown to black, uniloculate, immersed in the host becoming erumpent when mature. Paraphyses hyaline, septate, cylindrical, ends rounded, up to 40 μm long, 3–4 μm wide. Conidiogenous cells hyaline, cylindrical, holoblastic. Conidia (17-)21–27(−31) × 12.5–15.