In the study, we aim to detail anti-metastatic effects and molecular mechanisms of baicalein on HCC cells. Methods: The anti-metastatic effect of baicalein was determined using wound healing assay and transwell invasive model. find more The expression of MMP-2, MMP-9 and u-PA mRNA and protein in MHCC97H cells was determined by quantitative RT-PCR and western blot. The activity of MMP-2, MMP-9 and u-PA was determined by zymography.
The expression of TIMP-1 and TIMP-2 was determined by Western blot. The expression of MEK1 and ERK1/2 was measured by Western blot. Expression Plasmids (pcDNA3.1(+)-MEK1)were constructed and transfected Results: The migration and invasion of MHCC97H cells were markedly suppressed by baicalein in a dose-dependent manner. MMP-2, -9 and u-PA have been considered to be important in cancer cell invasion and metastasis because they play important Opaganib nmr roles in the degradation of the ECM. In our study, we found that treatment with baicalein on MHCC97H for 24h resulted in a decrease in MMP-2, -9 and u-PA expression, as well as proteinase activity. Meanwhile, the expression
of TIMP-1 and TIMP-2 were increased in a dose-dependent fashion. Thus, the anti-metastatic effect of baicalein on MHCC97H cells is correlated to proteinases and their inhibitors. Moreover, ERK pathway is closely correlated with synthesis of proteinases and their inhibitors. In our study, we found that
baicalein treatment decreased the levels of phosphorylation of MEK1 and ERK1/2. Overexpression of MEK1 partially blocked anti-metastatic effects of baicalein. Conclusion: Baicalein preferentially inhibits HCC invasion through inhibition of ERK pathway and by regulating synthesis of proteinases and their inhibitors. Acknowledgements: this website Supported by a grant from Program for changjiang Scholars and Innovative Research Team in University (PCSIRT: 1171). Key Word(s): 1. Baicalein; 2. HCC; 3. ERK pathway; 4. ECM; Presenting Author: WEILI HUANG Additional Authors: XIAOHUI GUAN Corresponding Author: WEILI HUANG Affiliations: Department of Digestion, Affiliated Hospital of Beihua University Objective: To investigate the relationship between the contents of β-catenin in cell nucleus of gastric cancer tissue and sFas in blood plasma and the degree of hyperplasia and infiltration of gastric cancer cells.