4%) and T54S (4.9%). These variants were often present in combinations, with V36M+R155K (10.4%), T54S+R155K (2.2%) and V36M+T54S+R155K (1.2%) occurring most often. The combination of V36M+T54S was seen only in the triple variant, V36M+T54S+R155K. Q80 substitutions were seen in 34.2% of
GT1a patients and 1.3% of GT1b patients. Q80K, the most frequent substitution, was seen in 32.3% of GT1a patient samples, but only 0.1% of GT1b samples. The combination of Q80K+R155K was seen in GT1 a samples only, at a frequency of 6.0%. Conclusions: The analysis of TVR and BoC RAVs among the first 1500 samples tested is consistent with that of the first 500. Our findings demonstrate a higher prevalence of HCV PI RAVs among GT1a versus GT1b samples. For TPV and BOC RAVs Decitabine purchase this is consistent with a higher genetic barrier for GT1b viruses. Q80K substitutions were frequently observed, which may significantly impact treatment decisions utilizing SMV. These RAVs were also observed during clinical trials. These findings support the consideration of baseline NS3/4A resistance testing prior
to the initiation of SMVcontaining regimens. Disclosures: click here Sunny S. Choe – Employment: Monogram Biosciences Joseph M. Volpe – Employment: Monogram Biosciences Jacqueline D. Reeves – Employment: Monogram Biosciences Wei Huang – Employment: monogram biosciences Mojgan Haddad – Employment: Monogram Biosciences Christos J. Petropoulos – Employment: Monogram Biosciences, LabCorp; Management Position: Monogram Biosciences, LabCorp; Patent Held/Filed: Monogram Biosciences, LabCorp; Stock Shareholder: LabCorp Charles M. Walworth – Employment: Monogram Biosciences BACKGROUND: In 2009, IL28B genotype (gt) was identified as the 上海皓元 strongest baseline predictor of peginterferon+ribavirin (PR) response in HCV1. In 2013, a novel dinucleotide variant in interferon-lambda-4 (IFNL4, ss469415590,
ΔG/TT), in high linkage disequilibrium (LD) with IL28B polymorphism, was proposed to be the causal variant. IFNL4 gt was a better predictor of sustained virological response (SVR). We have performed the first independent validation study of the association between IFNL4 gt, IL28B gt, and PR treatment outcomes in Australian HCV1/3 patients. METHODS: HCV1/3 patients who received PR were included. IL28B (rs12979860) and IFNL4 (ss469415590) gts were determined (ĪaqMan allelic discrimination kit, custom designed primers where testing unsuccessful). IFNL4 gt was correlated with rapid virological response (RVR) and SVR, and compared to IL28B gt using logistic regression modeling and LD calculation. RESULTS: 270 PR treatment patients were included: 56% HCV1, 44% HCV3. Self-reported ethnicity for HCV1 was 79% Caucasian and 20% Asian, and for HCV3 was 90% Caucasian and 3% Asian. Overall SVR rates were 50% (HCV1) and 82% (HCV3). IFNL4 gt could not be determined in 31 patients, and DNA re-extraction +/- concentration was required.