asiaticum to mimic that in F. graminearum by swapping the promoters. Additionally, the different functional requirement of MAT1-1-1
and MAT1-1-3 for sexual development between homothallic F. graminearum and S. macrospora implies that some of the regulatory networks controlled by MAT proteins may not be Roxadustat conserved among filamentous ascomycetes. This research was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ008210), Rural Development Administration, Republic of Korea, and by the Agricultural Research Center program of the Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea. “
“We report the enhanced bactericidal activity of ofloxacin in drug-containing Eudragit E100® dispersions (EuCl-OFX) against Pseudomonas aeruginosa and Seliciclib chemical structure the effect of the cationic polymer on bacterial membrane. Organisms treated
with EuCl-OFX showed changes in cell morphology, altered outer membrane (OM) and cytoplasm with low electrodensity areas. Zeta potential of bacterial surface was shifted to positive. Sensitization to lytic agents was also observed. A profound effect on bacterial size, granularity and membrane depolarization was found by flow cytometry. Cultures exposed to drug-free polymer also showed some damaged bacterial membranes, but there was no significant cell death. Inhibition of P. aeruginosa by EuCl-OFX may involve surface effect and, to some extent, permeation effect. The cationic polymer act to mitigate the electronegativity of cell surface in the process MYO10 of disorganizing the OM, rendering it more permeable to antibiotic. In addition, cytoplasmic membrane depolarization turns bacterial cell more vulnerable. The effects on membranes combined with the mechanism of action of quinolone explain the improved bactericidal action
exhibited by EuCl-OFX. The behavior described for Eudragit E100® against P. aeruginosa may be a useful tool to broaden the spectrum of antibiotics whose clinical use is limited by the impermeability of the bacterial OM. Infections caused by Pseudomonas aeruginosa are difficult to treat due to the intrinsic resistance of this microorganism to multiple classes of antimicrobial agents and to the ability to acquire induced resistance during therapy (Aloush et al., 2006; Bertino, 2009; Giamarellou, 2010). Strategies devised to reduce microbial multiresistance include control measures for the use of antibiotics, detection of genetic resistance mechanisms, a search for new synthetic or natural substances with antimicrobial activity or those contributing to enhancing the action of known antibiotics as well as the development of new strategies of drug delivery (Wright, 2010; Moellering, 2011). The resistance of P. aeruginosa to antibiotics is primarily attributed to reduced outer membrane (OM) permeability (Nikaido, 1989; Hancock, 1998; Lambert, 2002). Strategies to minimize the low OM-permeability of P.